Coordinates: 43°32′00″N 80°13′25″W / 43.53333°N 80.22361°W / 43.53333; -80.22361

The University of Guelph is a comprehensive public research university in Guelph, Ontario, Canada. It was established in 1964 after the amalgamation of Ontario Agricultural College (1874), the MacDonald Institute (1903), and the Ontario Veterinary College (1922), and has since grown to an institution of almost 30,000 students (including those at the Humber campus, Ridgetown campus, off-campus degree enrolments, diploma enrolments and part-time students) and employs 830 full-time faculty (academic staff) as of fall 2019. It offers 94 undergraduate degrees, 48 graduate programs, and 6 associate degrees in many different disciplines.

The veterinary medicine program at the University of Guelph was ranked No. 1 in Canada and No. 5 in the world in 2021. The University of Guelph is ranked No. 4 in Canada in Maclean's "University Rankings 2021" in the Comprehensive category, No. 1 in student satisfaction and No. 1 in research dollars. The university conducts a significant degree of research and offers a wide range of undergraduate, graduate and professional degrees. It is given top marks for student satisfaction among medium-sized universities in Canada by The Globe and Mail. The Ontario Agricultural College is ranked No. 1 in Canada for Agricultural Science, and helps put the University of Guelph 18th in the world for Food Science and Technology. It has held these rankings with its reputation, innovative research-intensive programs, and lively campus life cited as particular strengths. According to the Journal of Hospitality & Tourism Research, the university's Hospitality and Tourism Management program has Canada's highest research index. The University of Guelph has also been ranked 50th by Times Higher Education in its list of the top 100 universities under 50 years old. The university has a key focus on life science and has ranked 76–100 in the world by ARWU.